Crosslinking of CD44 on human osteoblastic cells upregulates ICAM‐1 and VCAM‐1

FEBS Letters

Nakano, Kazuhisa¹  Fujii, Yuko¹  Fujii, Koichi¹  Tanaka, Yoshiya¹ 

[1]The First Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi, Kitakyushu 807-8555, Japan

关键词: Osteoblast;    Osteoclast;    CD44;    Hyaluronan;    Intercellular adhesion molecule-1;    Vascular cell adhesion molecule-1;   

DOI  :  10.1016/S0014-5793(03)00182-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cell adhesion of osteoblasts and osteoclastic precursors of hematopoietic origin is a prerequisite for osteoclast maturation. We have investigated the relevance of osteoblast–matrix binding and regulation of adhesion molecules to this process. Human osteoblastic cells highly expressed CD44, a major receptor for hyaluronan present in the surrounding bone matrix. Crosslinking of CD44 on osteoblastic cells with specific antibodies augmented the expression of intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1. Hyaluronan, the major ligand of CD44, also up-regulated ICAM-1 expression. Stimulation of CD44 on osteoblastic cells amplified their adhesion to monocytic cells through ICAM-1 and VCAM-1. These results suggest that such crosstalk among distinct adhesion molecules may be relevant to bone metabolism, including osteoclastogenesis.

【 授权许可】

Unknown