Potato tuber phospholipids contain colneleic acid in the 2‐position

FEBS Letters

Williams, Todd D.²  Welti, Ruth¹  Fauconnier, Marie-Laure³  Marlier, Michel³ 

[1] Division of Biology, Ackert Hall, Kansas State University, Manhattan, KS 66506, USA;Mass Spectrometry Laboratory, University of Kansas, Lawrence, KS 66045, USA;Unité de Chimie Générale et Organique, Faculté Universitaire des Sciences Agronomiques de Gembloux, Passage des Déportés 2, B-5030 Gembloux, Belgium

关键词: Colneleic acid;    Potato;    Oxylipin;    Phospholipid;    Mass spectrometry;    ESI;    electrospray ionization;    fwhh;    full width at half-height;    MS;    mass spectrometry;    MS/MS;    tandem mass spectrometry;    PC;    phosphatidylcholine;    PE;    phosphatidylethanolamine;    PI;    phosphatidylinositol;    TOF;    time of flight;   

DOI  :  10.1016/S0014-5793(03)00171-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Colneleic acid (9-[1′(E),3′(Z)-nonadienyloxy]-8(E)-nonenoic acid) is produced from linoleic acid by the sequential action of 9-lipoxygenase and divinyl ether synthase. We demonstrate that a small fraction of the colneleic acid in potato tubers is esterified in phospholipids. This colneleic acid was released by chemical hydrolysis and a phospholipase A₂, but not by a lipase with 1-acyl specificity. Phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol contain molecular species with nominal masses consistent with identification as palmitoyl,colneleoyl species. Exact mass analysis of its fragments confirmed the identity of palmitoyl,colneloyl phosphatidylinositol. To our knowledge, this work represents the first identification of a colneleoyl phospholipid.

【 授权许可】

Unknown