Specific conjugation of DNA binding proteins to DNA templates through thiol–disulfide exchange

FEBS Letters

Vorob'eva, Olga V.¹  Metelev, Valeri G.¹  Oretskaya, Tatiana S.¹  Kubareva, Elena A.²  Romanenkov, Andrey S.¹ 

[1] Chemical Department, M.V. Lomonosov Moscow State University, Vorob'evi gori, Moscow 119992, Russia;A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Vorob'evi gori, Moscow 119992, Russia

关键词: Modified oligonucleotide;    Conjugation;    Thiol–disulfide exchange;    Phosphoryldisulfide linkage;    DNA methyltransferase;    pss;    phosphoryldithio linkage;    M.SsoII;    (cytosine-5)-DNA-methyltransferase from Shigella sonnei;   

DOI  :  10.1016/S0014-5793(03)00122-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The double-stranded oligodeoxyribonucleotides with single internucleotide disulfide linkages were successfully used for covalent trapping of cysteine containing protein. In particular, an efficient conjugation of DNA methyltransferase SsoII to sequence-specific decoys was demonstrated. The obtained results assume that synthetic oligodeoxyribonucleotides bearing a new trapping site can be used as new tools to study and manipulate biological systems.

【 授权许可】

Unknown