期刊论文详细信息
FEBS Letters | |
Specific conjugation of DNA binding proteins to DNA templates through thiol–disulfide exchange | |
Vorob'eva, Olga V.1  Metelev, Valeri G.1  Oretskaya, Tatiana S.1  Kubareva, Elena A.2  Romanenkov, Andrey S.1  | |
[1] Chemical Department, M.V. Lomonosov Moscow State University, Vorob'evi gori, Moscow 119992, Russia;A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Vorob'evi gori, Moscow 119992, Russia | |
关键词: Modified oligonucleotide; Conjugation; Thiol–disulfide exchange; Phosphoryldisulfide linkage; DNA methyltransferase; pss; phosphoryldithio linkage; M.SsoII; (cytosine-5)-DNA-methyltransferase from Shigella sonnei; | |
DOI : 10.1016/S0014-5793(03)00122-4 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The double-stranded oligodeoxyribonucleotides with single internucleotide disulfide linkages were successfully used for covalent trapping of cysteine containing protein. In particular, an efficient conjugation of DNA methyltransferase SsoII to sequence-specific decoys was demonstrated. The obtained results assume that synthetic oligodeoxyribonucleotides bearing a new trapping site can be used as new tools to study and manipulate biological systems.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020312760ZK.pdf | 243KB | download |