Novel two‐color fluorescence probe with extreme specificity to bovine serum albumin

FEBS Letters

Ercelen, Sebnem¹  Klymchenko, Andrey S.¹  Demchenko, Alexander P.¹ 

[1] TUBITAK Research Institute for Genetic Engineering and Biotechnology, 41470 Gebze-Kocaeli, Turkey

关键词: Fluorescence probe;    Ligand binding;    Bovine serum albumin;    Polarity sensing;    Two-color ratiometric dye;    3-Hydroxychromone;    BSA;    bovine serum albumin;    ESIPT;    excited-state intramolecular proton transfer;    FA;    2-(6-diethylaminobenzo[b]furan-2-yl)-3-hydroxychromone;   

DOI  :  10.1016/S0014-5793(03)00116-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report on strong, highly specific and stochiometric binding to bovine serum albumin of novel fluorescence probe FA, 2-(6-diethylaminobenzo[b]furan-2-yl)-3-hydroxychromone, that exhibits a very characteristic two-band fluorescence spectrum. Both absorption band and two fluorescence bands of FA are very sensitive to non-covalent interactions in the immediate environment of the probe. Multiparametric analysis of this spectroscopic information allows us to conclude that the binding site is characterized by very low polarity, high extent of screening from aqueous environment and unusually high electronic polarizability. The latter suggests the proximal location of probe FA to the aromatic amino acid residues in the binding site. The new probe can be proposed for the study of interaction of ligands and drugs of different nature with serum albumins.

【 授权许可】

Unknown