Functional role of sperm surface glutathione S‐transferases and extracellular glutathione in the haploid spermatozoa under oxidative stress

FEBS Letters

Hemachand, T.¹  Shaha, Chandrima¹ 

[1] National Institute of Immunology, Aruna Asaf Ali Road, New Delhi 110067, India

关键词: Glutathione S-transferase;    Oxidative stress;    Sperm;    GST;    glutathione S-transferase;    GSH;    reduced glutathione;    Δψ m;    mitochondrial membrane potential;    ROS;    reactive oxygen species;    HNE;    4-hydroxynonenal;    PnA;    cis-parinaric acid;    JC-1;    5;    5′;    6;    6′-tetrachloro-1;    1′;    3;    3′-tetraethylbenzimidazole carbocyanide iodide;   

DOI  :  10.1016/S0014-5793(03)00103-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

On the sperm surface, glutathione S-transferases (GSTs) exist as oocyte binding proteins but their detoxification function in this unique cell type is not known. Using H₂O₂- and 4-hydroxynonenal-induced sperm dysfunction models, this study demonstrates that the sperm surface GSTs are able to use extracellular reduced glutathione to inhibit the loss of functional competence of goat spermatozoa; however, in the presence of GST inhibitors, they are unable to do so. In the context of susceptibility of spermatozoa to oxidative stress, this finding that strategically located sperm surface GSTs are important for maintaining the functional competence of sperm is relevant to studies on male infertility.

【 授权许可】

Unknown