Further enhancement of baculovirus insecticidal efficacy with scorpion toxins that interact cooperatively

FEBS Letters

Gurevitz, Michael²  Inceoglu, Bora¹  Gershburg, Eduard³  Rivkin, Hadassah³  Regev, Avital³  Chejanovsky, Nor³  Hammock, Bruce D.¹ 

[1] Department of Entomology, University of California, Davis, CA 95616 USA;Department of Plant Sciences, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Ramat-Aviv 69978 Tel-Aviv, Israel;Entomology Department, Institute of Plant Protection, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel

关键词: Heliothis virescens;    Helicoverpa armigera;    Spodoptera littoralis;    Recombinant baculovirus;    Cooperative anti-insect scorpion toxin;    AcMNPV;    Autographa californica nucleopolyhedrovirus;    PIBS;    polyhedral inclusion bodies;    ET50;    effective time to paralysis;   

DOI  :  10.1016/S0014-5793(03)00104-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have studied whether the cooperative insecticidal effect of certain scorpion toxin pairs, namely either a combination of excitatory and depressant, or alpha and depressant scorpion toxins, would improve the efficacy of Autographa californica nucleopolyhedrovirus (AcMNPV) over a virus expressing only a single toxin, towards Heliothis virescens, Helicoverpa armigera, and Spodoptera littoralis larvae. The best result was achieved by combined expression of the excitatory toxin, LqhIT1, and the depressant toxin, LqhIT2, that provided an ET₅₀ value of 46.9 h on H. virescens neonates, an improvement of 40% over the efficacy of wild-type AcMNPV, and of 18% and 22% over baculoviruses that express each of the toxins independently. These results demonstrate that significant improvement in efficacy of recombinant baculoviruses is obtainable with toxins that exhibit a cooperative effect, and may contribute to employ baculoviruses to replace hazardous chemicals in insect control.

【 授权许可】

Unknown