Inhibition of the plant cytokinin transduction pathway by bacterial histidine kinase inhibitors in Catharanthus roseus cell cultures

FEBS Letters

Papon, Nicolas²  Crèche, Joël²  Clastre, Marc¹  Rideau, Marc¹  Gantet, Pascal²  Chénieux, Jean-Claude¹ 

[1] Unité sous Contrat reconnue par l'INRA ‘Facteurs de transcription et ingénierie métabolique végétale’, UFR des Sciences et Techniques et UFR des Sciences Pharmaceutiques, Université de Tours, Tours, France;Plant Molecular Biology and Biochemistry Department, EA 2106, Plant Biocompounds and Biotechnology, Faculty of Pharmacy, Université de Tours, 31 avenue Monge, 37200 Tours, France

关键词: Anilide derivative;    Cytokinin;    Histidine kinase;    Response regulator;    Catharanthus roseus;   

DOI  :  10.1016/S0014-5793(03)00102-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We describe the isolation of two Catharanthus roseus cDNAs encoding proteins putatively involved in the final steps of a ‘histidine-to-aspartate’ phosphorelay in cytokinin (CK) signaling. The expression of one of these genes, CrRR1, was specifically up-regulated by CKs in C. roseus cell suspensions. We used this system as a biological model to test the activity of bacterial histidine kinase inhibitors. Our data demonstrate that these inhibitors are active on the CK transduction pathway and represent powerful chemical tools to study hormone signal transduction in plants. Moreover, these data suggest a strong conservation of functional features between prokaryotic and plant signaling pathways utilizing histidine kinases.

【 授权许可】

Unknown