A novel testis‐specific RAG2‐like protein, Peas: its expression in pachytene spermatocyte cytoplasm and meiotic chromatin

FEBS Letters

Sutou, Shizuyo¹  Mitsui, Youji¹  Ohinata, Yasuhide¹ 

[1] National Institute of Advanced Industrial Science and Technology (AIST), Central 6, Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan

关键词: Peas;    RAG2;    Meiosis;    DNA double strand break;    Homologous recombination;   

DOI  :  10.1016/S0014-5793(03)00036-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report a novel gene Peas that constitutes an overlapping gene complex in mammalian genome. We have cloned human and mouse Peas cDNAs (hPEAS/mPeas) and analyzed their tissue and stage-specific expressions. Peas protein contains six repeated kelch motifs, structurally similar to RAG2, a V(D)J recombination activator, and is evolutionarily conserved among mammals, birds, insects, and nematodes. Northern, RNA in situ hybridization and immunohistochemical analyses showed that mPeas is specifically transcribed in testis, particularly in pachytene spermatocytes in which it is localized to the cytoplasm and meiotic chromatin. It is suggested that Peas may be involved in meiotic recombination process.

【 授权许可】

Unknown