Expression of syntaxin 1C, an alternative splice variant of HPC‐1/syntaxin 1A, is enhanced by phorbol‐ester stimulation in astroglioma: participation of the PKC signaling pathway 1

FEBS Letters

Nakayama, Takahiro³  Mikoshiba, Katsuhiko²  Akagawa, Kimio³  Yamamori, Tetsuo¹ 

[1] Division of Speciation Mechanisms, National Institute for Basic Biology, Aichi 444-8585, Japan;RIKEN Brain Science Institute, Developmental Brain Science Group, Laboratory for Developmental Neurobiology, Saitama 351-0198, Japan;Department of Physiology, Kyorin University School of Medicine, Tokyo 181-8611, Japan

关键词: Syntaxin 1;    Astroglioma;    Phorbol-ester;    Protein kinase C;    Protein kinase C inhibitor;    SNARE;    soluble N-ethylmaleimide-sensitive fusion protein (NSF) attachment protein receptor;    TMD;    transmembrane domain;    DMEM;    Dulbecco's modified Eagle's medium;    PBS;    phosphate-buffered saline;    PMA;    phorbol 12-myristate 13-acetate;    PKC;    protein kinase C;    GF;    GF109203X;   

DOI  :  10.1016/S0014-5793(03)00015-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Syntaxin 1C is an alternative splice variant of HPC-1/syntaxin 1A; the latter participates in neurotransmitter release and is assigned to the gene domain responsible for Williams’ syndrome (WS). It is expressed in the soluble fraction extracted from human astroglioma cell lines T98G and U87MG. Quantitative immunoblot and indirect immunofluorescence analyses revealed that the expression of syntaxin 1C was upregulated by phorbol 12-myristate 13-acetate (PMA), but not by forskolin. A protein kinase C (PKC) inhibitor suppressed this enhancement. These results suggest that syntaxin 1C expression is regulated via the PKC signal pathway. This is the first report of a signal transduction system that directly affects the expression of syntaxin protein.

【 授权许可】

Unknown