Ubiquitin binding proteins protect ubiquitin conjugates from disassembly

FEBS Letters

Hendil, Klavs B²  Gordon, Colin¹  Hartmann-Petersen, Rasmus² 

[1] MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK;August Krogh Institute, University of Copenhagen, Universitetsparken 13, DK-2100 Copenhagen O, Denmark

关键词: Ubiquitin;    Proteasome;    Deubiquitinating enzyme;    Ubiquitin-C-terminal hydrolase;    Ubiquitin pathway associated domain;    Ubiquitin interaction motif;    AMC;    7-amino-4-methylcoumarin;    DUB;    deubiquitinating enzyme;    GST;    glutathione S-transferase;    Ubal;    ubiquitin aldehyde;    UBA;    ubiquitin pathway-associated domain;    UBP;    ubiquitin-specific protease;    UIM;    ubiquitin interaction motif;   

DOI  :  10.1016/S0014-5793(02)03874-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

As a step in their turnover proteins in eukaryotic cells are coupled to a small protein, ubiquitin, before they are recognised by 26S proteasomes and degraded. However, cells also contain many deubiquitinating enzymes, which can rescue proteins by cleaving off the ubiquitin chains. Here we report that three ubiquitin binding proteins, Rhp23, Dph1 and Pus1, from fission yeast can protect multiubiquitin conjugates against deubiquitination. This protection depends on the ubiquitin binding domains and may promote degradation of ubiquitinated proteins.

【 授权许可】

Unknown