Lcb4p sphingoid base kinase localizes to the Golgi and late endosomes

FEBS Letters

Hait, Nitai C¹  Dickson, Robert C¹  Lester, Robert L¹  Fujita, Katsuhide¹ 

[1] Department of Molecular and Cellular Biochemistry and Lucille P. Markey Cancer Center, University of Kentucky College of Medicine, Lexington, KY 40536, USA

关键词: Sphingosine kinase;    Sphingosine-1-phosphate;    Sphingolipid;    Phytosphingosine;    DHS;    dihydrosphingosine;    ER;    endoplasmic reticulum;    LCB;    long chain base;    LCBP;    long chain base phosphate;    PHS;    phytosphingosine;    PM;    plasma membrane;    PVC;    prevacuolar compartment;    SD;    defined medium;    YPD;    yeast extract;    peptone;    dextrose;   

DOI  :  10.1016/S0014-5793(02)03636-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sphingoid long chain base phosphates (LCBPs) regulate cell proliferation, survival and motility in mammals. To learn more about LCBPs in Saccharomyces cerevisiae, we determined the cellular location of Lcb4p, the major enzyme catalyzing LCBP synthesis. By indirect immunofluorescence microscopy and subcellular fractionation, Lcb4p localizes to the trans-Golgi network and late endosomes and cycles between these compartments. Lcb4p faces the cytosol and is probably bound to membranes by protein–protein interactions. These results indicate that LCBs made in the endoplasmic reticulum must transit to the Golgi to be converted into LCBPs, which must then return to the endoplasmic reticulum to be degraded.

【 授权许可】

Unknown