Identification of a novel RNA silencing suppressor, NSs protein of Tomato spotted wilt virus

FEBS Letters

Takeda, Atsushi¹  Mori, Masashi³  Kaido, Masanori¹  Nagano, Hideaki²  Mise, Kazuyuki¹  Okuno, Tetsuro¹  Tsuda, Shinya²  Sugiyama, Kazuhiko¹ 

[1] Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan;Virus Disease Laboratory, Department of Plant Pathology, National Agricultural Research Center, Tsukuba, Ibaraki 305-8666, Japan;Laboratory of Plant Molecular Genetics, Research Institute of Agricultural Resources, Ishikawa Agricultural College, 1-308, Suematsu, Nonoichi-machi, Ishikawa 921-8836, Japan

关键词: RNA silencing;    Suppressor;    Tospovirus;    Bunyaviridae;    NSs;    p19;    dsRNA;    double-stranded RNA;    GFP;    green fluorescent protein;    IR-PTGS;    inverted repeat transgene-induced post-transcriptional gene silencing;    PTGS;    post-transcriptional gene silencing;    RdRP;    RNA-dependent RNA polymerase;    S-PTGS;    sense transgene-induced post-transcriptional gene silencing;    siRNAs;    small interfering RNAs;    TBSV;    Tomato bushy stunt virus;    TSWV;    Tomato spotted wilt virus;   

DOI  :  10.1016/S0014-5793(02)03632-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

RNA silencing or post-transcriptional gene silencing (PTGS) in plants is known as a defense system against virus infection. Several plant viruses have been shown to encode an RNA silencing suppressor. Using a green fluorescent protein-based transient suppression assay, we show that NSs protein of Tomato spotted wilt virus (TSWV) has RNA silencing suppressor activity. TSWV NSs protein suppressed sense transgene-induced PTGS but did not suppress inverted repeat transgene-induced PTGS. TSWV NSs protein is the first RNA silencing suppressor identified in negative-strand RNA viruses.

【 授权许可】

Unknown