In vivo and in vitro activation of temperature‐responsive plant map kinases

FEBS Letters

Sangwan, Veena¹  Dhindsa, Rajinder S¹ 

[1] Department of Biology, McGill University, 1205 Avenue Docteur Penfield, Montreal, QC, Canada H3A 1B1

关键词: Cold;    Heat;    Temperature;    MAPK;    Plant;    Alfalfa;    ERK;    extracellular signal-regulated kinase;    MAPK;    mitogen-activated protein kinase;    MBP;    myelin basic protein;   

DOI  :  10.1016/S0014-5793(02)03626-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Alfalfa cells possess two temperature-responsive Mitogen-Activated Protein Kinases (MAPKs), SAMK (Stress-Activated MAP Kinase) activated at 4°C and HAMK (Heat shock-Activated MAP Kinase) activated at 37°C. Both are inactive at 25°C. We show here that SAMK is activated when cells are transferred from 37°C to 25°C, and HAMK is activated when cells are transferred from 4°C to 25°C. Moreover, we show that heat activation of HAMK also occurs in cell-free extracts. We conclude that (i) SAMK or HAMK activation does not require a particular temperature but a relative temperature shift, and (ii) that either HAMK itself or one or more of its upstream activators can sense temperature change directly.

【 授权许可】

Unknown