A recessive mutant cell line with a constitutive IκB kinase activity

FEBS Letters

Miura, Hideyasu¹  Yamamoto, Naoki¹  Yamaoka, Shoji¹  Chinanonwait, Netnaphis¹ 

[1] Department of Molecular Virology, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8519, Japan

关键词: Recessive mutation;    NF-κB;    IKK activation;    EMSAs;    electrophoretic mobility shift assays;    IκBα;    I kappa B alpha;    IκBβ;    I kappa B beta;    IKK;    I kappa B kinase;    LPS;    lipopolysaccharide;    NEMO;    nuclear factor-kappa B essential modulator;    NF-κB;    nuclear factor-kappa B;   

DOI  :  10.1016/S0014-5793(02)03618-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To search for negative regulatory components of the NF-κB activation pathways, we mutagenized Rat-1 fibroblasts and established a stable mutant cell line with a constitutive NF-κB activity. This mutant cell line, designated as TK26, showed permanently elevated I kappa B kinase (IKK) activity and a genetically recessive phenotype revealed by somatic cell hybridization between TK26 and Rat-1. Our results suggested that lack of a negative regulation of IKK could lead to permanent NF-κB activation. The TK26 cell line will be useful to genetically identify a component necessary for keeping the IKK complex under an inactive form in resting cells.

【 授权许可】

Unknown