Amphioxus homologs of Go‐coupled rhodopsin and peropsin having 11‐cis‐ and all‐trans‐retinals as their chromophores

FEBS Letters

Terakita, Akihisa²  Koyanagi, Mitsumasa²  Shichida, Yoshinori²  Kubokawa, Kaoru¹ 

[1]Ocean Research Institute, University of Tokyo, Tokyo 164-8639, Japan

[2]Department of Biophysics, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan

关键词: Opsin;    Chromophore;    Amphioxus;    Retinal;    Peropsin;    Phylogenetic tree;    Gt;    transducin;    Gq;    Gq-type G protein;    Go;    Go-type G protein;    GPCR;    G protein-coupled receptor;    HPLC;    high-performance liquid chromatography;   

DOI  :  10.1016/S0014-5793(02)03616-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Because of low contents in the native organs and failure of the expression in cultured cells, the chromophore configurations of the pigments in Go-coupled opsin and peropsin groups in the opsin family are unknown. Here we have succeeded in expression of the amphioxus homologs of these groups in HEK293s cells and found that they can be regenerated with 11-cis- and all-trans-retinals, respectively. Light isomerized the chromophores of these opsins into the all-trans and 11-cis forms, respectively. The results strongly suggest that the physiological function of peropsin would be a retinal photoisomerase, while 11-cis configuration is necessary for the Go-coupled opsin groups.

【 授权许可】

Unknown