Local inhibition of Drosophila homeobox‐containing neural dorsoventral patterning genes by Dpp

FEBS Letters

Jeon, Sang-Hak¹  Han, Pyung-Lim¹  Kim, Sang Hee²  Kwon, Seung Hae¹  Oh, Chun Taek¹  Jeon, Kyoung Ja¹ 

[1] Department of Biological Sciences, Konkuk University, 1 Hwayang-Dong, Kwangjin-Gu, Seoul 143-701, South Korea;Department of Chemistry, Konkuk University, 1 Hwayang-Dong, Kwangjin-Gu, Seoul 143-701, South Korea

关键词: Dorsoventral patterning;    Central nervous system;    Homeodomain genes;    dpp;    Drosophila;   

DOI  :  10.1016/S0014-5793(02)03573-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An important step in Drosophila neurogenesis is to establish the neural dorsoventral (DV) patterning. Here we describe how dpp loss-of- and gain-of-function mutation affects the homeobox-containing neural DV patterning genes expressed in the ventral neuroectoderm. Ventral nervous system defective (vnd), intermediate neuroblast defective (ind), muscle-specific homeobox (msh), and orthodenticle (otd) genes participate in development of the central nervous system and peripheral nervous system, and encode homeodomain proteins. otd and msh genes were ectopically expressed in dpp loss-of-function mutation, but vnd and ind were not affected. However, when dpp was ectopically expressed in the ventral neuroectoderm by rho-GAL4/UAS-dpp system, it caused the repression of vnd, and msh expressions in ventral and dorsal columns of the neuroectoderm, respectively, but not that of ind. The later expression pattern of otd was also restricted by Dpp. The expression pattern of msh, vnd and otd in dpp loss-of-function and gain-of-function mutation indicates that Dpp activity does not reach to the ventral midline and it works locally to establish the dorsal boundary of the ventral neuroectoderm.

【 授权许可】

Unknown