Acidianus ambivalens type‐II NADH dehydrogenase: genetic characterisation and identification of the flavin moiety as FMN

FEBS Letters

Bandeiras, Tiago M¹  Teixeira, Miguel¹  Gomes, Cláudio M¹  Salgueiro, Carlos¹  Kletzin, Arnulf² 

[1] Instituto de Tecnologia Quı́mica e Biológica, Universidade Nova de Lisboa, Rua da Quinta Grande 6, Apt 127, 2780-156 Oeiras, Portugal;Institut für Biochemie, Medizinische Universität zu Lübeck, 23538 Lübeck, Germany

关键词: Type II NADH dehydrogenase;    Archaea;    Sulfolobales;    FMN;    31P NMR;   

DOI  :  10.1016/S0014-5793(02)03514-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The thermoacidophilic archaeon Acidianus ambivalens contains a monomeric 47 kDa type-II NADH dehydrogenase (NDH), which contains a covalently bound flavin. In this work, by a combination of several methods, namely ³¹P-nuclear magnetic resonance and fluorescence spectroscopies, it is proven that this enzyme contains covalent FMN, a novelty among this family of enzymes, which were so far thought to mainly have the flavin dinucleotide form. Discrimination between several possible covalent flavin linkages was achieved by spectral and fluorescence experiments, which identified an 8α-N(1)-histidylflavin-type of linkage. Analysis of the gene-deduced amino acid sequence of type-II NDH showed no transmembranar helices and allowed the definition of putative dinucleotide and quinone binding motifs. Further, it is suggested that membrane anchoring can be achieved via amphipatic helices.

【 授权许可】

Unknown