A heat‐activated MAP kinase in tomato: a possible regulator of the heat stress response

FEBS Letters

Ehness, Rainer¹  Vashista, Pooja¹  Roitsch, Thomas¹  Link, Vinzenz¹  Proels, Reinhard K¹  Sinha, Alok K¹  Hofmann, Markus G¹ 

[1] Julius von Sachs Institut für Biowissenschaften, Pharmazeutische Biologie, Julius von Sachs Platz 2, 87082 Würzburg, Germany

关键词: MAP kinase;    MAP kinase substrate;    Heat stress;    Heat shock factor;    Phosphorylation;   

DOI  :  10.1016/S0014-5793(02)03498-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Adaptation to elevated temperatures is of major importance for the survival of plants. The role of kinases in heat stress response was studied in tomato by in gel and in solution kinase assays using myelin basic protein as substrate. The application of heat stress in a naturally occurring temperature range resulted in a fast and transient activation of a 50 kDa mitogen-activated protein (MAP) kinase both in a photoautotrophic cell suspension culture and in leaves of mature plants. The heat activation of the MAP kinase was shown to be calcium-dependent. The specific phosphorylation of tomato heat stress transcription factor HsfA3 by a partially purified preparation of the heat-activated MAP kinase supports a physiological role of the identified kinase activity in transducing the heat stress signal.

【 授权许可】

Unknown