Response heterogeneity of human macrophages to ATP is associated with P2X7 receptor expression but not to polymorphisms in the P2RX7 promoter1

FEBS Letters

Li, C.M¹  Campbell, S.J³  Lammas, D.A¹  Kumararatne, D.S²  Hill, A.V.S³ 

[1] Division of Immunity and Infection, The Medical School, MRC Centre for Immune Regulation, University of Birmingham, Birmingham B15 2TT, UK;Department of Clinical Immunology, Addenbrookes Hospital, Cambridge CB2 2QQ, UK;Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, UK

关键词: P2X7;    Promoter;    Polymorphism;    ATP;    Cell lysis;    1513;    P2RX7;    P2X7 gene;    SNP;    single nucleotide polymorphism;    FACS;    flow cytometry staining;    IFNγ;    interferon gamma;    TB;    tuberculosis;   

DOI  :  10.1016/S0014-5793(02)03424-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A region 2 kb upstream of exon 1 of the P2X₇ gene was sequenced using DNA from nine healthy individuals who exhibited three different ATP response phenotypes (i.e. high, low and interferon gamma-inducible). Five single nucleotide polymorphisms were identified within the nine donor promoter sequences but none were associated with a specific ATP response phenotype. A P2X₇ loss of function polymorphism (1513 in exon 13) was also screened for within donor DNA but no response associations were identified. ATP response phenotype was positively associated with P2X₇ receptor expression, as assessed by flow cytometry, but not with any identified receptor or promoter gene polymorphisms.

【 授权许可】

Unknown