4α‐Phorbol negates the inhibitory effects of phorbol‐12‐myristate‐13‐acetate on human cilia and alters the phosphorylation of PKC

FEBS Letters

Mwimbi, Xowi K.M.S¹  Green, Michael¹  Muimo, Richmond¹  Sijumbila, Gibson¹  Treharne, Kate J¹  Mehta, Anil¹ 

[1] Tayside Institute of Child Health, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, UK

关键词: Lung;    Phorbol ester;    Ciliary beat frequency;    M199;    medium 199;    PKC;    protein kinase C;    CBF;    ciliary beat frequency;    PMA;    phorbol-12-myristate-13-acetate;    4α-PHR;    4α;    9α;    12β;    13α;    20-pentahydrotiglia-1;    6-dien-3-one;    4α-PMA;    inactive PMA control;    BIMI;    bisindolylmaleimide I;    MyrPKCI;    myristoylated peptide inhibitor of PKC;    HNE;    human nasal epithelial cells;   

DOI  :  10.1016/S0014-5793(02)03358-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In medium 199, ciliary beat frequency (CBF) in human nasal epithelium declines to 60% of baseline by 2 h and 1 nM phorbol-12-myristate-13-acetate (PMA) doubles the rate of decline by activating protein kinase C (PKC). We find that a reported negative control for PMA, 4α-phorbol (1 pM–1 nM)±1 nM PMA, not only maintains CBF at baseline, but arrests a pre-existing PMA-induced decline in CBF and alters the profile of multiple phosphorylated PKC species. Thus, 4α-phorbol not only potently prevents PMA from inhibiting CBF but also has potent effects on the phosphorylation of PKC.

【 授权许可】

Unknown