B plexins activate Rho through PDZ‐RhoGEF

FEBS Letters

Olivo, Cristina²  Driessens, Mariette H.E²  Collard, John G²  Nagata, Koh-ichi¹  Inagaki, Masaki¹ 

[1] Division of Biochemistry, Aichi Cancer Center Research Institute, 1-1 Chikusa-ku, Nagoya, Aichi 464-8181, Japan;Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands

关键词: Plexin;    Semaphorin;    Rho;    PDZ;    Guanine nucleotide exchange factor;    Axon guidance;    DH;    Dbl homology;    GEF;    guanine nucleotide exchange factor;    PDZ;    PSD-95/Dlg/ZO-1;    PH;    pleckstrin homology;    RGS;    regulator of G protein signaling;    S1P;    sphingosine-1-phosphate;   

DOI  :  10.1016/S0014-5793(02)03323-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Plexins are receptors for the repulsive axon guidance molecules semaphorins. Previously, we have shown that plexin-B1 binds activated Rac, but that clustering of plexin-B1 causes Rho activation, resulting in stress fiber formation. Using the yeast two-hybrid system, we found that the C-terminus of B plexins interacted directly with Rho-specific exchange factors, via their PDZ domain. Mutation of the carboxy-terminal amino acids of plexin-B1 or coexpression of a dominant negative PDZ-RhoGEF abrogated the ability of plexin-B1 to cause stress fiber formation. Our results demonstrate a role for PDZ-RhoGEF in B plexin-mediated activation of Rho/Rho kinase signaling, implicated in the regulation of axon guidance and cell migration.

【 授权许可】

Unknown