Fluorescence of native and carotenoid‐depleted LH2 from Chromatium minutissimum, originating from simultaneous two‐photon absorption in the spectral range of the presumed (optically ‘dark’) S1 state of carotenoids

FEBS Letters

Kummrow, A³  Scheer, H⁵  Lokstein, H²  Leupold, D³  Moskalenko, A⁴  Voigt, B³  Krikunova, M³  Rini, M³  Razjivin, A¹ 

[1] A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Moscow 119899, Russia;Institut für Biologie/Pflanzenphysiologie, Humboldt-Universität zu Berlin, Unter den Linden 6, D-10099 Berlin, Germany;Max-Born-Institut für Nichtlineare Optik und Kurzzeitspektroskopie, Max-Born-Strasse 2a, D-12489 Berlin, Germany;Institute of Fundamental Problems of Biology, Russian Acadamy of Science, Puschino, Moscow, Region 142292, Russia;Botanisches Institut der Universität München, Menzinger Strasse 67, D-80683, München, Germany

关键词: Carotenoid-less;    Excitation energy transfer;    Rhodopsin;    Two-photon excited fluorescence;    Chromatium minutissimum;    (B)Chl;    (bacterio)chlorophyll;    EET;    excitation energy transfer;    IC;    internal conversion;    LH2;    peripheral purple bacterial light-harvesting complex;    NIR;    near infra-red;    TPA;    (simultaneous) two-photon absorption;    TPF;    (simultaneous) two-photon excited fluorescence;   

DOI  :  10.1016/S0014-5793(02)03315-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Native and carotenoid-depleted peripheral purple bacterial light-harvesting complex (LH2) were investigated by simultaneous two-photon excited (between 1300–1500 nm) fluorescence (TPF). TPF results from direct bacteriochlorophyll excitation in both samples. The spectral position of the 2A_g ⁻ state of rhodopsin is indicated by a diminuition of the bacteriochlorophyll TPF in native LH2. In conclusion, comparison to carotenoid-depleted samples is a conditio sine qua non for unambiguous interpretation of similar experiments.

【 授权许可】

Unknown