Glucocorticoid inhibits expression of V‐1, a catecholamine biosynthesis regulatory protein, in cultured adrenal medullary cells

FEBS Letters

Kurahashi, Yusuke³  Honma, Takao²  Ueno, Susumu¹  Yanagihara, Nobuyuki¹  Iwanaga, Toshihiko⁵  Hatada, Risa³  Ohizumi, Yasushi⁴  Hiwatashi, Yusuke⁴  Yamakuni, Tohru⁴  Yanase, Haruko⁵ 

[1] Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan;Department of Applied Biochemistry, School of Engineering, Tokai University, 1117 Kitakaname, Hirasawa, Kanagawa 259-1292, Japan;Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194-8511, Japan;Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-ku, Sendai 980-8578, Japan;Laboratory of Anatomy, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan

关键词: V-1 protein;    Catecholamine biosynthesis;    Adrenal chromaffin cells;    Glucocorticoid;   

DOI  :  10.1016/S0014-5793(02)03293-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

V-1 acts as a positive and coordinate regulator of gene expression of catecholamine biosynthetic enzymes in PC12D cells. The present study was conducted to investigate the mechanism controlling expression of V-1 in the adrenal gland. Immunohistochemical analysis demonstrated that noradrenergic chromaffin cells more highly expressed V-1 than adrenergic chromaffin cells preferentially expressing the glucocorticoid receptor in rat adrenal glands. Western blotting showed that in cultured bovine adrenal medullary cells, dexamethasone, a synthetic glucocorticoid, inhibited expression of V-1, and that this inhibition was prevented by RU-486, a glucocorticoid receptor antagonist. These results suggest that adrenal expression of V-1 is differentially controlled by glucocorticoids through the specific receptor, and that thereby V-1 regulates catecholamine biosynthesis in a catecholaminergic phenotype-dependent manner.

【 授权许可】

Unknown