Ectodomain shedding of furin: kinetics and role of the cysteine‐rich region

FEBS Letters

Bissonnette, Lyne¹  Lavigne, Pierre¹  Longpré, Jean-Michel¹  Leduc, Richard¹  Charest, Gabriel¹  Denault, Jean-Bernard¹ 

[1] Department of Pharmacology, Faculty of Medicine, Université de Sherbrooke, Sherbrooke, QC, Canada J1H 5N4

关键词: SPC1;    Furin;    Ectodomain shedding;    Convertase;    Cysteine-rich region;    ACE;    angiotensin-converting enzyme;    Amc;    7-amido-4-methylcoumarin;    CRR;    cysteine-rich region;    ER;    endoplasmic reticulum;    SPC;    subtilisin pro-protein convertase;    TACE;    proTNF-α-converting enzyme;    TGN;    trans-Golgi network;    TMD;    trans-membrane domain;    TNFR;    tumor necrosis factor receptor;   

DOI  :  10.1016/S0014-5793(02)03249-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Furin, a member of the subtilisin-like pro-protein convertase family, is a type I membrane protein that undergoes ectodomain shedding. Metabolic labeling of cells stably expressing furin demonstrated that the shed form of furin is detected after 30 min. Moreover, sequence analysis revealed that specific residues of the cysteine-rich region of furin aligned with those of tumor necrosis factor receptor, which is also shed. Introduction within furin's cysteine-rich region of mutations that impair TNFR1 shedding also abolished furin shedding. Our results show that shedding of furin occurs rapidly and further suggest that specific cysteine residues may impart a conformation to the enzyme, thereby affecting its susceptibility to proteolysis.

【 授权许可】

Unknown