A single nucleotide polymorphism in the alternative oxidase gene among rice varieties differing in low temperature tolerance

FEBS Letters

Abe, Fumitaka³  Miura, Kiyoyuki¹  Toriyama, Kinya³  Saito, Koji² 

[1] Hokuriku Department of Rice Research, National Agricultural Research Center, 1-2-1, Inada, Joetsu, Niigata 943-0193, Japan;National Agricultural Research Center for Hokkaido Region, Hitsujigaoka 1, Toyohira, Sapporo, Hokkaido 062-8555, Japan;Laboratory of Plant Breeding and Genetics, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan

关键词: Alternative oxidase;    Isoforms;    Single nucleotide polymorphism;    Low temperature tolerance;    Oryza sativa L;   

DOI  :  10.1016/S0014-5793(02)03218-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Alternative oxidase (AOX) is encoded in a multigene family, and multiple isoforms have been observed in various plant species. We found for the first time an allelic variation in the same AOX locus. On SDS–gel blots of callus protein of rice (Oryza sativa L.), varieties without the QTL for low temperature tolerance showed a 32-kDa AOX band, whereas those with the QTL showed a 34-kDa band. The variation was attributed to the substitution of Lys⁷¹ for Asn⁷¹ caused by a single nucleotide polymorphism between alleles of OsAOX1a, and was tightly linked to the presence of the QTL.

【 授权许可】

Unknown