The COOH‐terminus of parathyroid hormone‐related protein (PTHrP) interacts with β‐arrestin 1B 1

FEBS Letters

Conlan, Lindus A¹  Martin, T.John¹  Gillespie, Matthew T¹ 

[1]St. Vincent's Institute of Medical Research, 9 Princes Street, Fitzroy, Vic. 3065, Australia

关键词: β-Arrestin;    Parathyroid hormone-related protein;    Endosome;    Yeast two-hybrid;    DNA-AD;    DNA activation domain;    DNA-BD;    DNA binding domain;    GST;    glutathione-S-transferase;    GPCR;    G-protein-coupled receptor;    HA;    hemagglutinin;    MAPK;    mitogen-activated protein kinase;    NLS;    nuclear localization sequence;    PCR;    polymerase chain reaction;    PTH;    parathyroid hormone;    PTHrP;    parathyroid hormone-related protein;   

DOI  :  10.1016/S0014-5793(02)03164-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Parathyroid hormone-related protein (PTHrP) has a diverse range of proposed biological activities participating in both extracellular and intracellular signaling. In order to identify candidate protein effectors, yeast two-hybrid screens were conducted using mature human PTHrP (residues 1–141) and the COOH-terminus (residues 107–141). Both PTHrP baits interacted with a β-arrestin 1B fragment, an important component of G-protein-coupled receptor desensitization and MAPK signaling. Co-immunoprecipitation, in vitro binding assays and colocalization experiments confirmed this interaction in human cells and this required residues 122–141 of PTHrP. These findings suggest that β-arrestin 1 acts as an effector for a novel function of PTHrP in cytoplasm.

【 授权许可】

Unknown