A role for the octameric ring protein, Translin, in mitotic cell division

FEBS Letters

Ishida, Reiko³  Shionoiri, Chieko³  Okado, Haruo²  Kasai, Masataka³  Sato, Hiroko³  Aoki, Katsunori¹ 

[1] Department of Hematology (Internal Medicine), The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan;Department of Neurobiology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu, Tokyo 183, Japan;Department of Immunology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162, Japan

关键词: Translin;    Chromosome segregation;    Cytokinesis;   

DOI  :  10.1016/S0014-5793(02)03095-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The octameric ring protein, Translin, demonstrates marked similarities to the family of helicase enzymes regarding its quaternary organization and dimerization of subunits. Here we show that the level of Translin closely parallels the proliferative state in various cell types. Expression is periodic during the cell cycle, with protein synthesis becoming maximal in the S and mitosis phases, consistent with a role in cell division. Moreover, induced overexpression of Translin was found to accelerate cell proliferation. Confocal microscopic analysis revealed that Translin is localized at the centrosomes at prophase and the mitotic spindle at metaphase, then translocating to the spindle midbodies during cytokinesis. This novel localization is attributable to specific interactions with microtubules of the mitotic spindles, and especially γ-tubulin. The results suggest that Translin participates in processes ensuring the segregation of chromosomes and cytokinesis.

【 授权许可】

Unknown