Enterohemolysin operon of Shiga toxin‐producing Escherichia coli: a virulence function of inflammatory cytokine production from human monocytes

FEBS Letters

Zhang, Hui-Min¹  Wakisaka-Saito, Noriko¹  Taneike, Ikue¹  Yamamoto, Tatsuo¹ 

[1] Division of Bacteriology, Department of Infectious Disease Control and International Medicine, Niigata University Graduate School of Medical and Dental Sciences, 757 Ichibanchou, Asahimachidori, Niigata, Japan

关键词: Enterohemolysin operon;    Hemolysis;    Interleukin-1β;    Human monocyte;    mRNA expression;    Shiga toxin-producing Escherichia coli;    STEC;    Shiga toxin-producing Escherichia coli;    Hly;    hemolysin;    IL-1β;    interleukin-1β;    TNF-α;    tumor necrosis factor-α;    HUS;    hemolytic uremic syndrome;    RT-PCR;    reverse transcription-polymerase chain reaction;    Stx;    Shiga toxin;   

DOI  :  10.1016/S0014-5793(02)03027-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Shiga toxin-producing Escherichia coli (STEC) is associated with hemolytic uremic syndrome (HUS). Although most clinical isolates of STEC produce hemolysin (called enterohemolysin), the precise role of enterohemolysin in the pathogenesis of STEC infections is unknown. Here we demonstrated that E. coli carrying the cloned enterohemolysin operon (hlyC, A, B, D genes) from an STEC human strain induced the production of interleukin-1β (IL-1β) through its mRNA expression but not tumor necrosis factor-α from human monocytes. No IL-1β release was observed with an enterohemolysin (HlyA)-negative, isogenic E. coli strain carrying a mutation in the hlyA gene. The data suggest that enterohemolysin, a pore-forming toxin, induces the production of IL-1β, which is one of serum risk markers for HUS.

【 授权许可】

Unknown