In diatoms, a transthylakoid proton gradient alone is not sufficient to induce a non‐photochemical fluorescence quenching

FEBS Letters

Lavaud, J¹  Etienne, A.-L¹  Rousseau, B¹ 

[1] Laboratoire ‘Organismes Photosynthétiques et Environnement’, UMR CNRS 8543, Ecole Normale Supérieure, 46 rue d'Ulm, 75230 Paris Cedex 05, France

关键词: Diatom;    Diadinoxanthin;    Diatoxanthin;    Non-photochemical quenching;    Xanthophyll cycle;    Phaeodactylum tricornutum;    Chl;    chlorophyll;    CL;    continuous light regime (16 h light/8 h dark per day);    DCCD;    N;    N′-dicyclohexyl-carbodiimide;    DD;    diadinoxanthin;    DT;    diatoxanthin;    DTT;    dithiothreitol;    IL;    intermittent light regime (5 min light per hour);    LHC;    light-harvesting complex;    NPQ;    non-photochemical fluorescence quenching;    PS I and II;    photosystem I and II;    ΔpH;    transthylakoid proton gradient;   

DOI  :  10.1016/S0014-5793(02)02979-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Non-photochemical fluorescence quenching (NPQ) in diatoms is associated with a xanthophyll cycle involving diadinoxanthin (DD) and its de-epoxidized form, diatoxanthin (DT). In higher plants, an obligatory role of de-epoxidized xanthophylls in NPQ remains controversial and the presence of a transthylakoid proton gradient (ΔpH) alone may induce NPQ. We used inhibitors to alter the amplitude of ΔpH and/or DD de-epoxidation, and coupled NPQ. No ΔpH-dependent quenching was detected in the absence of DT. In diatoms, both ΔpH and DT are required for NPQ. The binding of DT to protonated antenna sites could be obligatory for energy dissipation.

【 授权许可】

Unknown