Stress‐induced premature senescence in BJ and hTERT‐BJ1 human foreskin fibroblasts

FEBS Letters

Chainiaux, Florence¹  Remacle, José¹  de Magalhães, João Pedro¹  Toussaint, Olivier¹ 

[1]Research Unit on Cellular Biology, Department of Biology, University of Namur (FUNDP), Rue de Bruxelles 61, 5000 Namur, Belgium

关键词: Cellular senescence;    Fibroblast;    Telomere;    Telomerase;    H2O2;    UVB;    SIPS;    stress-induced premature senescence;    SA β-gal;    senescence-associated β-galactosidase;    HDF;    human diploid fibroblast;    PD;    population doubling;    TRF;    terminal restriction fragment;    t-BHP;    tert-butylhydroperoxide;   

DOI  :  10.1016/S0014-5793(02)02973-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To test the involvement of the telomeres in the senescent phenotype, we used telomerase-immortalized human foreskin fibroblasts (hTERT-BJ1). We exposed hTERT-BJ1 and parental BJ cells to either UVB or H₂O₂ subcytotoxic stress(es). Both cell lines developed biomarkers of replicative senescence: loss of replicative potential, increase in senescence-associated β-galactosidase activity, typical senescence-like morphology, overexpression of p21^WAF-1 and p16^INK-4a, and decreased level of the hyperphosphorylated form of pRb. Telomere shortening was slightly higher under stress for both BJ and hTERT-BJ1 but still much lower than that reported for other cell lines. We conclude that pathways alternative to telomere shortening must cause the appearance of the senescence phenotype.

【 授权许可】

Unknown