A cytochrome c encoded by the nar operon is required for the synthesis of active respiratory nitrate reductase in Thermus thermophilus

FEBS Letters

Cava, Felipe¹  Vallés, Cristina¹  Zafra, Olga¹  Caro, Eddy¹  Ramı́rez, Sandra¹  Berenguer, José¹  Moreno, Renata¹  Castán, Pablo¹ 

[1] Centro de Biologı́a Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientı́ficas, Universidad Autónoma de Madrid, Campus de Cantoblanco, 28049 Madrid, Spain

关键词: Nitrate respiration;    Thermophilic;    Cytochrome c;    Thermus;   

DOI  :  10.1016/S0014-5793(02)02953-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cytochrome c (NarC) is encoded as the first gene of the operon for nitrate respiration in Thermus thermophilus. NarC is required for anaerobic growth and for the synthesis of active nitrate reductase (NR). The α and δ subunits (NarG, NarJ) of the NR were constitutively expressed in narC::kat mutants, but NarG appeared in the soluble fraction instead of associated with the membranes. Our data demonstrate for NarC an essential role in the synthesis of active enzyme and for the attachment to the membrane of the respiratory NR from T. thermophilus.

【 授权许可】

Unknown