Murine peripherin gene sequences direct Cre recombinase expression to peripheral neurons in transgenic mice

FEBS Letters

Santha, Miklos²  Zvara, Agnes²  Zhou, Li³  Rowley, Daniel L³  Simonneau, Michel²  Donovan, David M³  Levacher, Béatrice²  Mi, Qing-Sheng¹  Népote, Virginie² 

[1] Laboratory of Immunology, Gerontology Research Center, IRP, NIA, NIH, 5600 Nathan Shock Drive, Baltimore, MD 21224-6825, USA;Neurogenetique, INSERM E9935, Hopital Robert Debre, 48 blvd Serurier, 75019 Paris, France;Transgenic and Knockout Facility Section, Gerontology Research Center, IRP, NIA, NIH, 5600 Nathan Shock Drive, Baltimore, MD 21224-6825, USA

关键词: ROSA26;    Dorsal root ganglion;    Sensory neuron;    Cre;    loxP;    Peripherin;   

DOI  :  10.1016/S0014-5793(02)02936-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Spatially and temporally regulated somatic mutations can be achieved by using the Cre/loxP recombination system of bacteriophage P1. To develop a cell type-specific system of gene targeting in the peripheral nervous system, we generated the transgenic mouse lines expressing Cre recombinase under the control of the mouse peripherin gene promoter. The activity of the Cre recombinase during embryonic development was examined by mating the peripherin-Cre transgenic mice to the knock-in Cre-mediated recombination reporter strain, R26R. Analysis of F1 embryos from this cross showed specific excision of loxP-flanked sequences in the dorsal root ganglia, trigeminal ganglia, and olfactory epithelium, in a pattern very similar to the expression of the endogenous mouse peripherin gene, and the previously reported peripherin-lacZ transgenic mice. Thus, the peripherin-Cre mouse described here will provide a valuable tool for Cre-loxP-mediated conditional expression in the peripheral nervous system.

【 授权许可】

Unknown