Hypoxia activates a background conductance in freshly isolated pulmonary arterial endothelial cells of the rat

FEBS Letters

Kozlowski, Roland Z¹  Hogg, Dayle S¹ 

[1] Department of Pharmacology, School of Medical Sciences, University of Bristol, University Walk, Bristol BS8 1TD, UK

关键词: Pulmonary artery;    Endothelial cell;    Non-selective cation channel;    Hypoxia;    DCB;    2′;    4′-dichlorobenzamil;    E m;    membrane potential;    HEPES;    N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid;    HPV;    hypoxic pulmonary vasoconstriction;    I −90;    mean current at −90 mV;    I NSC;    non-selective cation current;    I–V;    current–voltage;    PAEC;    pulmonary artery endothelial cells;   

DOI  :  10.1016/S0014-5793(02)02912-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Utilising the patch-clamp recording technique we have demonstrated for the first time the effects of hypoxia on the background current in pulmonary arterial endothelial cells. Electrophysiological studies revealed the presence of a novel oxygen-sensitive, non-selective cation conductance (I _NSC) in these cells. The inward component of I _NSC was significantly potentiated by hypoxia. Both the inward and outward components of I _NSC were inhibited by both La³⁺ and Gd³⁺. Hypoxic activation of I _NSC may provide an important Ca²⁺ influx pathway essential for the release of a pulmonary-selective vasoconstrictor pivotal to the sustained phase of hypoxic pulmonary vasoconstriction.

【 授权许可】

Unknown