The gene expression and deficiency phenotypes of Cockayne syndrome B protein in Caenorhabditis elegans

FEBS Letters

Koo, Hyeon-Sook²  Ahn, Byungchan¹  Lee, Myon Hee²  Choi, In Soon³ 

[1] Department of Microbiology and Genetic Engineering, College of Science, Ulsan University, Ulsan 680-749, South Korea;Department of Biochemistry, College of Science, Yonsei University, Seoul 120-749, South Korea;Department of Life Science, Silla University, Pusan 617-736, South Korea

关键词: Nucleotide excision repair;    UV sensitivity;    Transcription-coupled repair;    Caenorhabditis elegans;    CSB;    Cockayne syndrome B;    dsRNA;    double-stranded RNA;    GFP;    green fluorescent protein;    NER;    nucleotide excision repair;    ORF;    open reading frame;    RT-PCR;    reverse transcription-polymerase chain reaction;    RNAi;    RNA interference;    TCR;    transcription-coupled repair;    XP;    xeroderma pigmentosum;   

DOI  :  10.1016/S0014-5793(02)02880-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Caenorhabditis elegans Cockayne syndrome B protein homologue is encoded by 10 exons of the predicted open reading frame F53H4.1. The gene is expressed in germ cells and all somatic cells of the embryonic to adult stage. Although the gene expression was ubiquitous, its expression level was relatively higher in dividing cells and cells that play fundamental roles in essential physiological functions such as feeding, sensation, and reproduction. RNA interference of the gene hypersensitized C. elegans to UV radiation, as observed in enhanced germ cell proliferation arrest and apoptosis, and increased embryonic lethality, suggesting its role in nucleotide excision repair.

【 授权许可】

Unknown