TGF‐β1 and Smad4 overexpression induce a less invasive phenotype in highly invasive spindle carcinoma cells

FEBS Letters

Santibáñez, Juan Francisco¹  Martı́nez, Jorge¹  Quintanilla, Miguel² 

[1] Laboratorio de Biologia Celular, INTA, Universidad de Chile, Casilla 138, Santiago 11, Chile;Instituto de Investigaciones Biomédicas Alberto Sols, CSIC-UAM, 28029 Madrid, Spain

关键词: TGF-B1;    Smad4;    Spindle;    Cat C;   

DOI  :  10.1016/S0014-5793(02)02760-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have examined the effect of transforming growth factor β₁ (TGF-β₁) and overexpression of the Smad4 gene on the phenotype of Car C, a ras mutated highly malignant spindle carcinoma cell line. TGF-β₁-treated Car C cells overexpressing Smad4 spread with a flattened morphology with membrane ruffles abundant in vinculin and show a reduction in their invasive abilities. TGF-β₁ treatment and overexpression of Smad4 also enhanced the production of PAI-1 measured by the activation of the p3TP-lux reporter gene containing a PAI-1-related promoter. This activation was abolished with a dominant-negative Smad4 construct. These results lead us to conclude that both TGF-β₁ and Smad4 overexpression reduce the invasive potential of Car C cells, probably via the Smad pathway.

【 授权许可】

Unknown