Human septin–septin interaction: CDCrel‐1 partners with KIAA0202

FEBS Letters

Bläser, Susanne¹  Zgaga-Griesz, Andrea¹  Busse, Anja¹  Jersch, Katrin¹  Wunderle, Daniela¹  Zieger, Barbara¹  Hainmann, Ina¹ 

[1] Children's Hospital, University of Freiburg, Mathildenstr. 1, D-79106 Freiburg, Germany

关键词: Septin;    Yeast two-hybrid assay;    Protein interaction;    KIAA0202;    CDCrel-1;    cell division cycle related-1;    G3PDH;    glyceraldehyde-3-phosphate dehydrogenase;   

DOI  :  10.1016/S0014-5793(02)02749-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Septins are evolutionary conserved cytoskeletal GTPases forming heteropolymer complexes involved in cytokinesis and other cellular processes. CDCrel-1 (cell division cycle related-1) is a recently cloned and characterized human septin which is highly expressed in non-dividing cells, such as neurons. Using a yeast two-hybrid system we demonstrate that CDCrel-1 partners with another uncharacterized human septin, KIAA0202. The interaction of CDCrel-1 and KIAA0202 was confirmed in the human leukemia cell line K-562 using pull-down assays with a KIAA0202–glutathione S-transferase fusion protein and by immunoprecipitation of the CDCrel-1–KIAA0202 complex with an anti-KIAA0202 antibody. Expression studies of the two human septins revealed a concomitant expression of both proteins in certain cells.

【 授权许可】

Unknown