Degradation of human Aurora‐A protein kinase is mediated by hCdh1

FEBS Letters

Honda, Kei²  Urano, Takeshi²  Furukawa, Koichi²  Yamaguchi, Akio¹  Taguchi, Sei-ichi²  Sugiura, Kazumitsu² 

[1] First Department of Surgery, Fukui Medical University, 23-4 Shimoaizuki Matsuoka-chou, Yoshida-gun, Fukui 910-11 Japan;Department of Biochemistry II, Nagoya University School of Medicine, 65 Tsurumai-machi, Showa-ku, Nagoya 466-0065, Japan

关键词: Aurora-A;    Cdh1;    Cdc20;    KEN box;    Anaphase promoting complex/cyclosome;    APC/C;    anaphase promoting complex/cyclosome;    PTTG;    pituitary tumor transforming gene;    LLnL;    N-acetyl-Leu-Leu-norleucinal;    GST;    glutathione S-transferase;    DMEM;    Dulbecco's modified Eagle's medium;    FCS;    fetal calf serum;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/S0014-5793(02)02711-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human Aurora-A is related to a protein kinase originally identified by its close homology to Ipl1p from Saccharomyces cerevisiae and aurora from Drosophila melanogaster, which are key regulators of the structure and function of the mitotic spindle. We previously showed that human Aurora-A is turned over through the anaphase promoting complex/cyclosome (APC/C)–ubiquitin–proteasome pathway. The association of two distinct WD40 repeat proteins known as Cdc20 and Cdh1, respectively, sequentially activates the APC/C. The present study shows that Aurora-A degradation is dependent on hCdh1 in vivo, not on hCdc20, and that Aurora-A is targeted for proteolysis through distinct structural features of the destruction box, the KEN box motifs and its kinase activity.

【 授权许可】

Unknown