In situ visualization of caspase‐1‐like activity associated with promotion of hippocampal cell death

FEBS Letters

Nakamura, Takeshi¹  Kudo, Yoshihisa¹  Shoda, Takuji¹  Takahashi, Kenji¹  Nishii, Wataru¹  Matsumoto, Nagisa¹ 

[1] School of Life Science, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan

关键词: Caspase-1;    Cell death;    Fluorescent substrate;    Fluorescence resonance energy transfer;    Glutamate;    Hippocampus;    Visualization;    acrylodan;    6-acryloyl-2-dimethylaminonaphthalene;    FRET;    fluorescence resonance energy transfer;    HPLC;    high-performance liquid chromatography;    YVAD;    Tyr-Val-Ala-Asp;    Z-DEVD-AFC;    N α-bezyloxycarbonyl-Asp-Glu-Val-Asp-4-trifluoromethylcoumaryl-7-amide;    Z-YVAD-AFC;    N α-benzyloxycarbonyl-Tyr-Val-Ala-Asp-4-trifluoromethylcoumaryl-7-amide;   

DOI  :  10.1016/S0014-5793(02)02687-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To clarify the function of caspase-1-like proteases in neuronal cell death, it is important to be able to detect the activity in living organs by microscopic visualization. In the present study, we synthesized a novel fluorescent substrate sensitive to the caspase-1-like activity, which is easily introduced into cells constituting living organs by extracellular application. As a result, the substrate was shown to be useful in imaging the caspase-1-like activity in rat hippocampal slice cultures. After induction of cell death with glutamate, a significant increase in the activity was observed, especially in the pyramidal cells, suggesting the association of the activity with promotion of cell death.

【 授权许可】

Unknown