Leucyl‐tRNA synthetase from the extreme thermophile Aquifex aeolicus has a heterodimeric quaternary structure

FEBS Letters

Gouda, Masaki¹  Asahara, Haruichi¹  Nishikawa, Kazuya¹  Yokogawa, Takashi¹ 

[1] Department of Biomolecular Science, Faculty of Engineering, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan

关键词: Aminoacylation;    Leucyl-tRNA synthetase;    Heterodimer;    Quaternary structure;    Aquifex aeolicus;    aaRS;    aminoacyl-tRNA synthetase;    LeuRS;    leucyl-tRNA synthetase;    MetRS;    methionyl-tRNA synthetase;    tRNALeu(CAG);    leucine tRNA which have anticodon CAG;   

DOI  :  10.1016/S0014-5793(02)02675-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Class I aminoacyl-tRNA synthetases have been thought to be single polypeptide enzymes. However, the complete genome sequence of a hyper thermophile Aquifex aeolicus suggests that the gene for leucyl-tRNA synthetases (LeuRS) is probably split into two pieces (leuS and leuS′). In this research, each gene was separately cloned and overexpressed in Escherichia coli and the protein products were examined for LeuRS activity. Leucylation activity was detected only when both gene products coexisted. Gel filtration analysis showed that the active form of A. aeolicus LeuRS has a heterodimeric (α/β type) quaternary structure that is unique among class I aminoacyl-tRNA synthetases.

【 授权许可】

Unknown