Two distinct classes of FixJ binding sites defined by in vitro selection

FEBS Letters

Kahn, Daniel¹  Ferrières, Lionel¹ 

[1] Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, UMR 215 INRA-CNRS, Chemin de Borde-Rouge, P.O. Box 27, 31326 Castanet-Tolosan Cedex, France

关键词: FixJ;    Transcriptional regulator;    Binding site;    Two-component system;    Nitrogen fixation;   

DOI  :  10.1016/S0014-5793(02)02618-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The phosphorylated FixJ transcriptional activator is key to nitrogen fixation in Sinorhizobium meliloti, switching both the nifA and fixK genes on. Previously no consensus picture emerged concerning the nature of FixJ binding sites. Here we used in vitro DNA selection in order to systematically characterise FixJ binding sequences. This led to the definition of two classes of sites. Class I sites share the CTAAGTAGTTTCCC sequence found in the fixK promoter, whereas class II sites are defined by a GTAMGTAG consensus octamer. Our results indicate that FixJ∼P binds DNA following two distinct binding modes.

【 授权许可】

Unknown