Nuclear Apaf‐1 and cytochrome c redistribution following stress‐induced apoptosis

FEBS Letters

González de Buitrago, Gonzalo¹  Martı́nez-A, Carlos¹  Ruiz-Vela, Antonio¹ 

[1] Department of Immunology and Oncology, Centro Nacional de Biotecnologı́a/CSIC, Universidad Autónoma de Madrid, Campus de Cantoblanco, E-28049 Madrid, Spain

关键词: Apaf-1;    Caspase-9;    Cytochrome c;    Stress-induced apoptosis;    Subcellular localization;    Apaf-1;    apoptotic protease activating factor-1;   

DOI  :  10.1016/S0014-5793(02)02607-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Apoptotic protease activating factor-1 (Apaf-1) and cytochrome c are cofactors critical for inducing caspase-9 activation following stress-induced apoptosis. One consequence of caspase-9 activation is nuclear–cytoplasmic barrier disassembly, which is required for nuclear caspase-3 translocation. In the nucleus, caspase-3 triggers proteolysis of the caspase-activated DNA nuclease (CAD) inhibitor, causing CAD induction and subsequent DNA degradation. Here we demonstrate that apoptotic cells show perinuclear cytochrome c aggregation, which may be critical for nuclear redistribution of cytochrome c and Apaf-1. We thus indicate that the nuclear redistribution of these cofactors concurs with the previously reported caspase-9-induced nuclear disassembly, and may represent an early apoptotic hallmark.

【 授权许可】

Unknown