cDNA macroarray analysis of gene expression in synoviocytes stimulated with TNFα

FEBS Letters

Sugiyama, Tomoyasu¹  Sugano, Sumio²  Hio, Yuri¹  Suzuki, Yuzuru²  Irie, Ryotaro¹  Isogai, Takao¹  Ota, Toshio¹  Yamamoto, Jun-ichi¹  Masuho, Yasuhiko¹  Saito, Kaoru¹  Wakamatsu, Ai¹  Ishii, Shizuko¹  Nishikawa, Tetsuo¹  Otuki, Tetsuji¹ 

[1] Helix Research Institute, Inc., 1532-3 Yana, Kisarazu-shi, Chiba 292-0812, Japan;Laboratory of Genome Structure, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan

关键词: Macroarray;    Gene expression;    Tumor necrosis factor-α;    Synoviocyte;    Oligo-capping;    Rheumatoid arthritis;   

DOI  :  10.1016/S0014-5793(02)02588-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Gene expression of synoviocytes stimulated with tumor necrosis factor-α (TNFα) was studied by macroarray analysis to elucidate the cellular response and identify new biological functions of known and unknown genes. 10 035 cDNA clones were used to make cDNA macroarrays of representative genes. Synoviocytes expressed large amounts of fibronectin and collagen mRNA. Statistical analysis of the macroarray data revealed 26 genes, including six new genes, which underwent significant alteration of gene expression in response to TNFα stimulation. These findings suggest that the synoviocyte response to TNFα stimulation forms the basis of development of various aspects of the pathophysiology of rheumatoid arthritis.

【 授权许可】

Unknown