A novel positive tetracycline‐dependent transactivator (rtTA) variant with reduced background activity and enhanced activation potential

FEBS Letters

Gohla, Gabi¹  Schlüter, Gregor¹  Kämper, Martin R¹ 

[1] Institute of Human Genetics, University of Göttingen, Heinrich Düker Weg 12, D-37075 Göttingen, Germany

关键词: Doxycycline;    VP16;    Induction;    Tet-On;    rtTA;   

DOI  :  10.1016/S0014-5793(02)02587-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The tetracycline-controlled transcription system has become one of the most potent systems for experimental manipulations of transcription levels in vivo. Here we report on rtTA variants, which were generated by combining the existing positively regulated Tet repressor domains of rtTA and rtTA-M2 with a modified and multimerized minimal transactivation domain from VP16 (L-domain). A transactivator with multimerized L-domains shows drastically reduced background activity and enhanced transcriptional activation on different tetracycline-responsive promoters. The new rtTA variants require higher doses of doxycycline and display a more linear dose–response curve than the original rtTA or rtTA-M2 proteins.

【 授权许可】

Unknown