Thiolsubtilisin acts as an acetyltransferase in organic solvents

FEBS Letters

Tai, Dar-Fu¹  Liaw, Wen-Chen² 

[1] Department of Chemistry, National Dong-Hwa University, Hualien, Taiwan;Department of Chemical Engineering, National Yunlin University of Science and Technology, Yunlin, Taiwan

关键词: Thiolsubtilisin;    N-Acetyltransferase;    Catalytic triad;    Ethyl acetate;    Transacetylation;   

DOI  :  10.1016/S0014-5793(02)02562-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The catalytic mechanism of arylamine N-acetyltransferase has been proposed to involve Cys-His-Asp as its catalytic triad. Thiolsubtilisin, a chemically modified enzyme that has a catalytic triad of Cys-His-Asp at the active site, mimics the catalysis of arylamine N-acetyltransferase, serotonin N-acetyltransferase, histone N-acetyltransferase and amino acid N-acetyltransferase. Thiolsubtilisin not only can catalyze amino acid transacetylation, but is also able to catalyze amine transacetylation. Ethyl acetate was used as the acylating reagent to form N-acetyl amino acids and amines in organic solvents with moderate yield. Hence, these findings broaden our understanding of the structural features required for N-acetyltransferases activity as well as provide a structural relationship between cysteine protease and other N-acyltransferases.

【 授权许可】

Unknown