Sucrose metabolism: Anabaena sucrose‐phosphate synthase and sucrose‐phosphate phosphatase define minimal functional domains shuffled during evolution

FEBS Letters

Cumino, Andrea¹  Salerno, Graciela L.¹  Giarrocco, Laura¹  Curatti, Leonardo¹ 

[1] Centro de Investigaciones Biológicas, FIBA, Mar del Plata, Argentina

关键词: Cyanobacterium;    Sucrose-phosphate synthase;    Sucrose-phosphate phosphatase;    Sucrose synthase;    Modular arrangement;    Phylogenetic analysis;    GTD;    glucosyltransferase domain;    PHD;    phosphohydrolase domain;    SBP;    sucrose biosynthesis-related proteins;    SPP;    sucrose-phosphate phosphatase;    SPS;    sucrose-phosphate synthase;    SuS;    sucrose synthase;    The prefixes indicate: An-;    Anabaena sp. PCC7120;    An7119-;    Anabaena sp. PCC7119;    At-;    Arabidopsis thaliana;    Mt-;    Medicago truncatula;    Os-;    Oryza sativa;    Np-;    Nostoc punctiforme;    PmMED-;    Prochlorococcus marinus MED4;    PmMIT-;    P. marinus MIT9313;    St-;    Solanum tuberosum;    Sm-;    Synechococcus marinus WH8102;    Sy-;    Synechocystis sp. PCC6803;    Zm-;    Zea mays;   

DOI  :  10.1016/S0014-5793(02)02516-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Based on the functional characterization of sucrose biosynthesis related proteins [SBP: sucrose-phosphate synthase (SPS), sucrose-phosphate phosphatase (SPP), and sucrose synthase (SuS)] in Anabaena sp. PCC7120 and sequence analysis, we have shown that SBP are restricted to cyanobacterium species and plants, and that they are multidomain proteins with modular architecture. Anabaena SPS, a minimal catalytic SPS unit, defines a glucosyltransferase domain present in all SPSs and SuSs. Similarly, Anabaena SPP defines a phosphohydrolase domain characteristic of all SPPs and some SPSs. Phylogenetic analysis points towards the evolution of modern cyanobacterial and plant SBP from a bidomainal common ancestral SPS-like gene.

【 授权许可】

Unknown