Distal regulatory regions restrict the expression of cis‐linked genes to the tapetal cells

FEBS Letters

de Oliveira, Dulce E.²  Franco, Luciana O.²  Sachetto-Martins, Gilberto²  de O. Manes, Carmem-Lara²  Hamdi, Said¹ 

[1] Departments of Molecular and Plant Genetics, Flanders Interuniversity Institute for Biotechnology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium;Laboratório de Genética Molecular Vegetal, Departamento de Genética, Universidade Federal do Rio de Janeiro, C.P. 68011, Rio de Janeiro 21941-970, Brazil

关键词: Distal element;    Gene regulation;    Pollenin;    Tapetum;    Arabidopsis thaliana;    Atgrp;    glycine-rich protein gene of Arabidopsis thaliana;    LPB;    lipid protein body;    LCR;    locus control region;    uidA;    β-glucuronidase gene from Escherichia coli;   

DOI  :  10.1016/S0014-5793(02)02557-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The oleosin glycine-rich protein genes Atgrp-6, Atgrp-7, and Atgrp-8 occur in clusters in the Arabidopsis genome and are expressed specifically in the tapetum cells. The cis-regulatory regions involved in the tissue-specific gene expression were investigated by fusing different segments of the gene cluster to the uidA reporter gene. Common distal regulatory regions were identified that coordinate expression of the sequential genes. At least two of these genes were regulated spatially by proximal and distal sequences. The cis-acting elements (122 bp upstream of the transcriptional start point) drive the uidA expression to floral tissues, whereas distal 5′ upstream regions restrict the gene activity to tapetal cells.

【 授权许可】

Unknown