Identification of ten exon deleted ERβ mRNAs in human ovary, breast, uterus and bone tissues: alternate splicing pattern of estrogen receptor β mRNA is distinct from that of estrogen receptor α

FEBS Letters

Baldwin, Kate¹  Abraham, Jessy¹  Poola, Indra¹ 

[1] Department of Anatomy, Howard University College of Medicine, Washington, DC 20059, USA

关键词: Exon deletion variant;    Splice targeted primer approach;    Estrogen receptor α;    Alternative splicing;    Sequence of exon deletions;    Estrogen receptor β;    Human ovary;    Human breast;    Human uterus;    Human bone and tissue specific alternate splicing;   

DOI  :  10.1016/S0014-5793(02)02521-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Four different human tissues and breast cancer cell lines were screened to identify exon deletion variant transcripts of estrogen receptor β (ERβ) by reverse transcription-polymerase chain reaction using the ‘splice targeted primer approach’ that amplifies each category of exon deleted variants as a separate gene population. A total of 10 different variant mRNAs that have deletions in various combination of exons were identified by sequence analysis. They were exon 2Δ; exons 2 and 5–6Δ; exon 3Δ; exon 4Δ; exon 5Δ; exons 5 and 2Δ; exon 6Δ; exons 6 and 2Δ; exons 6, 2–3Δ; and exons 5–6Δ. In some cases, deletion of an exon appears to be associated with a mutation of a specific base. Although ERα and ERβ are highly homologous, have identical exon and functional domain organization, exhibit similar ligand-binding profiles and interact with identical DNA response elements, the sequence of exon skipping in ERβ pre-mRNA appears to be distinct from that of ERα mRNA. Furthermore, results described here also suggest that alternate splicing of ERβ mRNA is tissue specific. The presence of a ERβ variant profile together with other ER isoforms in a tissue may have functional implications in binding and response to a particular ligand.

【 授权许可】

Unknown