Intracellular calcium mobilization induces period genes via MAP kinase pathways in NIH3T3 cells

FEBS Letters

Naruse, Yoshihisa¹  Oh-hashi, Kentaro¹  Tanaka, Masaki¹ 

[1]Department of Anatomy and Neurobiology, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamikyo-ku, Kyoto 602-0841, Japan

关键词: Thapsigargin;    Ca2+;    mPer1;    mPer2;    Mitogen-activated protein kinase;    NIH3T3 cell;    BAPTA-AM;    1;    2-bis-(o-aminophenoxy)ethane-N;    N;    N′;    N′-tetraacetic acid tetra-(acetoxymethyl)-ester;    CREB;    cAMP responsive element binding protein;    Cry;    cryptochrome;    Erk;    extracellular signal-regulated kinase;    MAP kinase;    mitogen-activated protein kinase;    Per;    period;    PKC;    protein kinase C;    PMA;    phorbol 12-myristate 13-acetate;    SCN;    suprachiasmatic nucleus;   

DOI  :  10.1016/S0014-5793(02)02510-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mammalian period genes have a pivotal role in generating circadian rhythms and are rapidly induced by several stimuli in mammalian cells. In the present study, we revealed that treatment with thapsigargin significantly induced transcripts of mouse period 1 and 2 (mPer1 and mPer2) but not mPer3 among circadian related genes in NIH3T3 cells. Thapsigargin-induced mPer1 and mPer2 mRNA expressions took distinct signaling pathways from protein kinase C and cAMP, but were partially inhibited by inhibitors of MEK1 and p38 mitogen-activated protein kinase, respectively. Thus, the present study suggested that intracellular calcium is one of multiple signaling stimuli triggering mPer gene expression in NIH3T3 cells.

【 授权许可】

Unknown