FEBS Letters | |
The SUPERMAN protein is an active repressor whose carboxy‐terminal repression domain is required for the development of normal flowers | |
Ohme-Takagi, Masaru1  Ohta, Masaru1  Matsui, Kyoko1  Hiratsu, Keiichiro1  | |
[1] Gene Discovery Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, Tsukuba 305-8566, Japan | |
关键词: Active repressor; Floral development; Cell proliferation; SUPERMAN; Arabidopsis; EAR; ERF-associated amphiphilic repression; SUP; SUPERMAN; GAL4DB; yeast GAL4 DNA-binding domain; LUC; luciferase; SUPRD; 175/204 repression domain of SUPERMAN; SUPΔRD; 1/174 region of SUPERMAN; AP3; APETALA3; | |
DOI : 10.1016/S0014-5793(02)02435-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
SUPERMAN was identified as a putative regulator of transcription that acts in floral development, but its function remains to be clarified. We demonstrate here that SUPERMAN is an active repressor whose repression domain is located in the carboxy-terminal region. Ectopic expression of SUPERMAN that lacked the repression domain resulted in a phenotype similar to that of superman mutants, demonstrating that the repression activity of SUPERMAN is essential for the development of normal flowers. Constitutive expression of SUPERMAN resulted in a severe dwarfism but did not affect cell size, indicating that SUPERMAN might regulate genes that are involved in cell division.
【 授权许可】
Unknown
【 预 览 】
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