Regulation of fibronectin alternative splicing by a basement membrane‐like extracellular matrix

FEBS Letters

Kornblihtt, Alberto R¹  Srebrow, Anabella¹  Blaustein, Matı́as¹ 

[1] Laboratorio de Fisiologı́a y Biologı́a Molecular, Departamento de Fisiologı́a, Biologı́a Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II (C1428EHA) Buenos Aires, Argentina

关键词: Alternative splicing;    Extracellular matrix;    Fibronectin;    FN;    Fibronectin;    ECM;    extracellular matrix;    TGF β1;    transforming growth factor β1;    EGF;    epidermal growth factor;    HGF/SF;    hepatocyte growth factor/scatter factor;   

DOI  :  10.1016/S0014-5793(02)02382-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hepatocytes are the source of plasma fibronectin (FN) which lacks the alternatively spliced EDI segment, distinctive of oncofetal FN. When hepatic or other epithelial cells are cultured on plastic, EDI inclusion is triggered. Here we report that EDI inclusion is inhibited when hepatic cells are cultured on a basement membrane-like extracellular matrix (ECM), demonstrating a new role for the ECM in the control of gene expression. The effect is duplicated by collagen IV and laminin but not by collagen I; is not observed with another alternatively spliced FN exon (EDII); and correlates with a decrease in cell proliferation, consistently with high EDI inclusion levels observed in many physiological and pathological proliferative processes.

【 授权许可】

Unknown